ARG62932
anti-CD80 antibody [16-10A1]
anti-CD80 antibody [16-10A1] for CyTOF®-candidate,Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse,Dog
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Hamster Monoclonal antibody [16-10A1] recognizes CD80 |
|---|---|
| 反应物种 | Ms, Dog |
| 应用 | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| 特异性 | The clone 16-10A1 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. |
| 宿主 | Hamster |
| 克隆 | Monoclonal |
| 克隆号 | 16-10A1 |
| 同位型 | IgG2 |
| 靶点名称 | CD80 |
| 抗原物种 | Mouse |
| 抗原 | Mouse CD80-transfected CHO cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1 |
应用说明
| 应用建议 |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Functional study: Functions as blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q00609 Mouse T-lymphocyte activation antigen CD80 |
|---|---|
| 基因名称 | Cd80 |
| 全名 | CD80 antigen |
| 背景介绍 | CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. |
| 生物功能 | Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. [UniProt] |
| 产品亮点 | Related products: CD80 antibodies; CD80 ELISA Kits; Anti-Hamster IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 33 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG62932 anti-CD80 antibody [16-10A1] FACS image
Flow Cytometry: Murine peripheral whole blood stained with ARG62932 anti-CD80 antibody [16-10A1] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
-
ARG62932 anti-CD80 antibody [16-10A1] FACS image
Flow Cytometry: Separation of murine CD80 positive monocytes (red-filled) from CD80 negative lymphocytes (black-dashed). Murine peritoneal fluid cells stained with ARG62932 anti-CD80 antibody [16-10A1] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
NF-kappaB2 is required for the control of autoimmunity by regulating the development of medullary thymic epithelial cells.
Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2.
Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis.
Induction of B7-1 in podocytes is associated with nephrotic syndrome.
Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract.
