ARG65433

anti-CD84 antibody [CD84.1.21] (FITC)

Datasheet

订购信息

包装

价格 (USD)

50 tests

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [CD84.1.21] recognizes CD84
反应物种	Hu
应用	FACS
特异性	The clone CD84.1.21 recognizes CD84, a single chain cell surface glycoprotein of 64-82 kDa, predominantly expressed B cells, monocytes, platelets and some T cells.
宿主	Mouse
克隆	Monoclonal
克隆号	CD84.1.21
同位型	IgG2a
靶点名称	CD84
抗原	CD84-transfected 300.19 cell line
偶联标记	FITC
別名	hCD84; Leukocyte differentiation antigen CD84; Hly9-beta; CD antigen CD84; LY9B; mCD84; Cell surface antigen MAX.3; SLAM family member 5; SLAMF5; Signaling lymphocytic activation molecule 5

应用说明

应用建议

应用	推荐稀释比
FACS	4 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 8832 Human CD84 Swiss-port # Q9UIB8 Human SLAM family member 5
基因名称	CD84
全名	CD84 molecule
背景介绍	CD84 is a highly glycosylated homophilic receptor of SLAM family. It is expressed on platelets and various types of leukocytes, especially following their activation. Ligation of CD84 leads to its phosphorylation on tyrosine residues within the cytoplasmic tail. These docking sites are recognized by downstream signaling molecules, such as phosphatase SHP-2 and adaptor protein SAP/SH2D1A. The function of CD84 has not been fully elucidated yet. Although predominantly activating receptor, its modulating activity was also demonstrated.
生物功能	Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	39 kDa
翻译后修饰	Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells. N-glycosylated.

检测图片 (2) Click the Picture to Zoom In

ARG65433 anti-CD84 antibody [CD84.1.21] (FITC) FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG65433 anti-CD84 antibody [CD84.1.21] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).
ARG65433 anti-CD84 antibody [CD84.1.21] (FITC) FACS image

Flow Cytometry: Separation of human CD177 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65433 anti-CD84 antibody [CD84.1.21] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).