ARG62937
anti-CD86 antibody [GL-1] (Functional Grade)
anti-CD86 antibody [GL-1] (Functional Grade) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Mouse
Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Functional grade and low endotoxin Rat Monoclonal antibody [GL-1] recognizes CD86 |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| 特异性 | The clone GL-1 reacts with CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | GL-1 |
| 同位型 | IgG2a |
| 靶点名称 | CD86 |
| 抗原物种 | Mouse |
| 抗原 | LPS-activated CBA/Cs mouse splenic B cells |
| 偶联标记 | Un-conjugated |
| 別名 | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | FACS: Mouse splenocytes |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P42082 Mouse T-lymphocyte activation antigen CD86 |
|---|---|
| 基因名称 | Cd86 |
| 全名 | CD86 antigen |
| 背景介绍 | CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. |
| 生物功能 | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG62937 anti-CD86 antibody [GL-1] (Functional Grade) FACS image
Flow Cytometry: Murine peritoneal fluid cells suspension stained with ARG62937 anti-CD86 antibody [GL-1] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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ARG62937 anti-CD86 antibody [GL-1] (Functional Grade) FACS image
Flow Cytometry: Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed). Murine peritoneal fluid cells suspension stained with ARG62937 anti-CD86 antibody [GL-1] (Functional Grade) at 0.6 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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