ARG22560
anti-CD88 / C5AR1 antibody [20/70] (PE)
anti-CD88 / C5AR1 antibody [20/70] (PE) for Flow cytometry and Mouse
概述
| 产品描述 | PE-conjugated Rat Monoclonal antibody [20/70] recognizes CD88 / C5AR1 This antibody recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Clone 20/70 has been reported to block the binding of the C5a to murine CD88, (Soruri et al. 2003). |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 20/70 |
| 同位型 | IgG2b |
| 靶点名称 | CD88 / C5AR1 |
| 抗原物种 | Mouse |
| 抗原 | RBL-2H3 transfected cells expressing murine CD88 / C5aR. |
| 偶联标记 | PE |
| 別名 | CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30993 Mouse C5a anaphylatoxin chemotactic receptor 1 |
|---|---|
| 基因名称 | C5ar1 |
| 全名 | complement component 5a receptor 1 |
| 生物功能 | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. [UniProt] |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042). Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338. |
