ARG62945

anti-CD9 antibody [IVA50]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
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概述

产品描述	Mouse Monoclonal antibody [IVA50] recognizes CD9
反应物种	Bov
应用	FACS, FuncSt, IP, WB
特异性	The clone IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
宿主	Mouse
克隆	Monoclonal
克隆号	IVA50
同位型	IgG2
靶点名称	CD9
抗原	Bovine thrombocytes.
偶联标记	Un-conjugated
別名	Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
FuncSt	Assay-dependent
IP	Assay-dependent
WB	1 µg/ml

应用说明

Functional studies: The clone IVA50 activates Bovine thrombocytes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from ascites by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 280746 Bovine CD9 Swiss-port # P30932 Bovine CD9 antigen
基因名称	CD9
全名	CD9 molecule
背景介绍	This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
生物功能	Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). [UniProt]
研究领域	Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
预测分子量	25 kDa
翻译后修饰	Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

检测图片 (3) Click the Picture to Zoom In

ARG62945 anti-CD9 antibody [IVA50] IP image

Immunoprecipitation: Biotin-labeled human platelets lysates was immunoprecipitated with 1) ARG62945 anti-CD9 antibody [IVA50], 2) anti-Human CD9 control antibody, and 3) negative control.
ARG62945 anti-CD9 antibody [IVA50] FACS image

Flow Cytometry: Bovine peripheral whole blood stained with ARG62945 anti-CD9 antibody [IVA50] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62945 anti-CD9 antibody [IVA50] FACS image

Flow Cytometry: Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed). Bovine peripheral whole blood stained with ARG62945 anti-CD9 antibody [IVA50] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

克隆号文献

Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.

ELISA / Cow

Colino J et al.
J Immunol., (2006)