ARG62943
anti-CD9 antibody [MEM-61]
anti-CD9 antibody [MEM-61] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-61] recognizes CD9 |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, FuncSt, IHC-P, WB |
| 特异性 | The clone MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-61 |
| 同位型 | IgG1 |
| 靶点名称 | CD9 |
| 抗原 | Pre-B cell line NALM-6. |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Under non-reducing condition. Functional studies: The clone MEM-61 induces FcγR-dependent platelet aggregation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-P: Prostate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD9 |
| 全名 | CD9 molecule |
| 背景介绍 | CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors. |
| 生物功能 | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. [UniProt] |
| 产品亮点 | Related products: CD9 antibodies; CD9 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Tools for studying Exosomes Detecting exosomal PD-L1 secreted by cancer cells |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
检测图片 (1) Click the Picture to Zoom In
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ARG62943 anti-CD9 antibody [MEM-61] CyTOF image
CyTOF: PBMC (after Ficoll-Paque separation) stained with ARG62943 anti-CD9 antibody [MEM-61] (Dy161).
克隆号文献
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.
WB / Human
Hypoxia-induced changes in the bioactivity of cytotrophoblast-derived exosomes.
WB / Human
Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal lipid raft microdomains influenced by the ΔΨm of subplasmalemmal mitochondria.
ICC/IF / Human
Role of interleukin 16 in multiple myeloma.
BL / Human
