ARG57542
anti-CD94 / KLRD1 antibody [13E3]
anti-CD94 / KLRD1 antibody [13E3] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [13E3] recognizes CD94 / KLRD1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 13E3 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD94 / KLRD1 |
| 抗原物种 | Human |
| 抗原 | Recombinant Human CD94 / KLRD1 (aa. 32-179) purified from E. coli |
| 偶联标记 | Un-conjugated |
| 別名 | KP43; CD antigen CD94; CD94; Natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KLRD1 |
| 全名 | killer cell lectin-like receptor subfamily D, member 1 |
| 背景介绍 | Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. [UniProt] |
| 预测分子量 | 21 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] FACS image
Flow Cytometry: PBMC cells stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 2 - 5 µg/10^6 cells (red). Secondary Ab: Goat anti-Mouse IgG antibody (Alexa fluor 488). Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] WB image
Western blot: 40 µg of K562, MCF7 and A549 cell lysates stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 1:1000 dilution.
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ARG57542 anti-CD94 / KLRD1 antibody [13E3] WB image
Western blot: 40 µg of NK-92 cell lysate stained with ARG57542 anti-CD94 / KLRD1 antibody [13E3] at 1:1000 dilution.
