ARG66139
anti-CD95 / Fas antibody (Biotin)
anti-CD95 / Fas antibody (Biotin) for ELISA,Western blot and Human
概述
| 产品描述 | Biotin-conjugated Rabbit Polyclonal antibody recognizes CD95 / Fas |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD95 / Fas |
| 抗原物种 | Human |
| 抗原 | E. coli derived recombinant Human CD95 / Fas. (MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS) |
| 偶联标记 | Biotin |
| 別名 | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by affinity chromatography. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| 基因名称 | FAS |
| 全名 | Fas cell surface death receptor |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| 生物功能 | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| 产品亮点 | Related products: CD95 antibodies; CD95 ELISA Kits; Anti-Rabbit IgG secondary antibodies; |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
检测图片 (4) Click the Picture to Zoom In
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ARG66139 anti-CD95 / Fas antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66139 anti-CD95 / Fas antibody (Biotin), under non-reducing conditions.
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ARG66139 anti-CD95 / Fas antibody (Biotin) standard curve image
Direct ELISA: ARG66139 anti-CD95 / Fas antibody (Biotin) at ~ 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66139 anti-CD95 / Fas antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human sFas Receptor stained with ARG66139 anti-CD95 / Fas antibody (Biotin), under reducing conditions.
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ARG66139 anti-CD95 / Fas antibody (Biotin) standard curve image
Sandwich ELISA: ARG66139 anti-CD95 / Fas antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66138 anti-CD95 / Fas antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
