ARG65390
anti-CD99 antibody [3B2/TA8] (FITC)
anti-CD99 antibody [3B2/TA8] (FITC) for Flow cytometry and Human
Cancer antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [3B2/TA8] recognizes CD99 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes. HLDA VI.; WS Code T 6T-097, BP 534 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3B2/TA8 |
| 同位型 | IgG2a |
| 靶点名称 | CD99 |
| 抗原物种 | Human |
| 抗原 | Human thymocytes |
| 偶联标记 | FITC |
| 別名 | 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD99 |
| 全名 | CD99 molecule |
| 背景介绍 | CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016] |
| 生物功能 | CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt] |
| 研究领域 | Cancer antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Extensively O-glycosylated. |
检测图片 (2) Click the Picture to Zoom In
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ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG65390 anti-CD99 antibody [3B2/TA8] (FITC).
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ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) FACS image
Flow Cytometry: Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).
克隆号文献
Lipid rafts and pseudotyping.
CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production.
