ARG40230

anti-CD99 antibody

anti-CD99 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CD99
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CD99
抗原物种 Human
抗原 Synthetic peptide derived from Human CD99.
偶联标记 Un-conjugated
別名 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

应用说明

应用建议
应用 推荐稀释比
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HUVEC
实际分子量 ~ 28 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4267 Human CD99

Swiss-port # P14209 Human CD99 antigen

基因名称 CD99
全名 CD99 molecule
背景介绍 CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
生物功能 CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
细胞定位 Membrane; Single-pass type I membrane protein. [UniProt]
预测分子量 19 kDa
翻译后修饰 Extensively O-glycosylated. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG40230 anti-CD99 antibody WB image

    Western blot: HUVEC cell lysate stained with ARG40230 anti-CD99 antibody.