ARG52240
anti-CDK5 antibody [1H3]
anti-CDK5 antibody [1H3] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody [1H3] recognizes CDK5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1H3 |
| 同位型 | IgG1 |
| 靶点名称 | CDK5 |
| 抗原物种 | Rat |
| 抗原 | Purified rat Cdk5 |
| 偶联标记 | Un-conjugated |
| 別名 | Cell division protein kinase 5; TPKII catalytic subunit; LIS7; PSSALRE; Serine/threonine-protein kinase PSSALRE; Cyclin-dependent-like kinase 5; EC 2.7.11.1; Tau protein kinase II catalytic subunit |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of Rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured Rat neurons and for immunohistochemistry on mouse brain tissue. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDK5 |
| 全名 | cyclin-dependent kinase 5 |
| 背景介绍 | The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology. |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity. Phosphorylation at Ser-159 is essential for maximal catalytic activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52240 anti-CDK5 antibody [1H3] WB image
Western Blot: rat caudate lysate showing specific immunolabeling of the ~28k Cdk5 protein stained with CDK5 antibody [1H3] (ARG52240).
