ARG66698
anti-CDK5R1 / p35 antibody
anti-CDK5R1 / p35 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDK5R1 / p35 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDK5R1 / p35 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 30-110 of Human CDK5R1 / p35. |
| 偶联标记 | Un-conjugated |
| 別名 | CDK5 activator 1; CDK5R; TPKII regulatory subunit; Cyclin-dependent kinase 5 activator 1; NCK5A; p25; p23; Cyclin-dependent kinase 5 regulatory subunit 1; p35nck5a; Tau protein kinase II 23 kDa subunit; CDK5P35; p35 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P61810 Rat Cyclin-dependent kinase 5 activator 1 Swiss-port # Q15078 Human Cyclin-dependent kinase 5 activator 1 |
|---|---|
| 基因名称 | CDK5R1 |
| 全名 | cyclin-dependent kinase 5, regulatory subunit 1 (p35) |
| 背景介绍 | The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008] |
| 生物功能 | p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. [UniProt] |
| 细胞定位 | CDK5R1, p35: Cell membrane; Lipid-anchor; Cytoplasmic side. Note=In the primary cortical neurons, p35 is present in the peripheries and nerve terminals. CDK5R1, p25: Nucleus. Cytoplasm, perinuclear region. Note=The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. [UniProt] |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo (By similarity). Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35. Ubiquitinated. Degradation of p35 by proteasome results in down-regulation of CDK5 activity. During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation. Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66698 anti-CDK5R1 / p35 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG66698 anti-CDK5R1 / p35 antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66698 anti-CDK5R1 / p35 antibody WB image
Western blot: K562 cell lysate stained with ARG66698 anti-CDK5R1 / p35 antibody at 1:1000 dilution.
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ARG66698 anti-CDK5R1 / p35 antibody WB image
Western blot: HeLa cell lysate stained with ARG66698 anti-CDK5R1 / p35 antibody at 1:1000 dilution.
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ARG66698 anti-CDK5R1 / p35 antibody WB image
Western blot: Rat brain lysate stained with ARG66698 anti-CDK5R1 / p35 antibody. The lane on the right is blocked with the synthetic peptide.
