ARG63903

Name: anti-CDKN2A / p16INK4a antibody
Brand: arigo Biolaboratories
SKU: ARG63903
Price: 499 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-CDKN2A / p16INK4a antibody

Datasheet

订购信息

包装

价格 (USD)

100 μg

499

50 μg

342

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Goat Polyclonal antibody recognizes CDKN2A / p16INK4a
反应物种	Hu
应用	IHC-P, WB
特异性	This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	CDKN2A / p16INK4a
抗原物种	Human
抗原	C-HARIDAAEGPSDIPD
偶联标记	Un-conjugated
別名	Alternative reading frame; CDK4I; P19ARF; P16INK4A; CDKN2; Tumor suppressor ARF; MTS-1; P14; P16; P19; Cyclin-dependent kinase inhibitor 2A; P16INK4; MLM; p14ARF; MTS1; INK4; TP16; P16-INK4A; CMM2; ARF; P14ARF; INK4A

应用说明

应用建议

应用	推荐稀释比
IHC-P	5 µg/ml
WB	0.1 - 1 µg/ml

应用说明

WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1029 Human CDKN2A Swiss-port # P42771 Human Cyclin-dependent kinase inhibitor 2A
基因名称	CDKN2A
背景介绍	This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Jul 2008]
产品亮点	Related products: CDKN2A antibodies; CDKN2A Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched
研究领域	Chondrogenesis Study antibody
预测分子量	14 kDa
翻译后修饰	Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its proteasomal degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation.

检测图片 (2) Click the Picture to Zoom In

ARG63903 anti-CDKN2A / p16INK4a antibody WB image

Western blot: 30 µg of 293T cell lysate stained with ARG63903 anti-CDKN2A / p16INK4a antibody at 1:500 dilution.
ARG63903 anti-CDKN2A / p16INK4a antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63903 anti-CDKN2A / p16INK4a antibody at 5 µg/ml dilution followed by AP-staining.