ARG64903
anti-CDKN2A / p16INK4a antibody
anti-CDKN2A / p16INK4a antibody for Western blot and Human
Chondrogenesis Study antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CDKN2A / p16INK4a |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize the pancreas isoform (NP_478104.1) only. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDKN2A / p16INK4a |
| 抗原物种 | Human |
| 抗原 | C-ILRKKGRLPGEFSE |
| 偶联标记 | Un-conjugated |
| 別名 | Alternative reading frame; CDK4I; P19ARF; P16INK4A; CDKN2; Tumor suppressor ARF; MTS-1; P14; P16; P19; Cyclin-dependent kinase inhibitor 2A; P16INK4; MLM; p14ARF; MTS1; INK4; TP16; P16-INK4A; CMM2; ARF; P14ARF; INK4A |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P42771 Human Cyclin-dependent kinase inhibitor 2A |
|---|---|
| 基因名称 | CDKN2A |
| 全名 | cyclin dependent kinase inhibitor 2A |
| 背景介绍 | This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. [UniProt] |
| 产品亮点 | Related products: CDKN2A antibodies; CDKN2A Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| 研究领域 | Chondrogenesis Study antibody |
| 预测分子量 | 14 kDa |
| 翻译后修饰 | Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its proteasomal degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation. |
检测图片 (1) Click the Picture to Zoom In
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ARG64903 anti-CDKN2A antibody WB image
Western Blot: HeLa lysate (35µg protein in RIPA buffer) stained with ARG64903 anti-p14ARF (isoform 3) antibody (1µg/ml)
