ARG54113
anti-CDX2 antibody
anti-CDX2 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Monkey
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CDX2 |
|---|---|
| 反应物种 | Hu, Ms, Mk |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | CDX2 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human CDX2 protein fragments expressed in E.coli |
| 偶联标记 | Un-conjugated |
| 別名 | CDX2/AS; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDX2 |
| 全名 | caudal type homeobox 2 |
| 背景介绍 | Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium.Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. |
| 生物功能 | Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Phosphorylation of Ser-60 mediates the transactivation capacity. |
检测图片 (3) Click the Picture to Zoom In
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ARG54113 anti-CDX2 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54113 anti-CDX2 antibody at 1:100 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54113 anti-CDX2 antibody WB image
Western blot: 30 µg of NIH3T3 cell lysate stained with ARG54113 anti-CDX2 antibody at 1:1000 dilution.
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ARG54113 anti-CDX2 antibody IP image
Immunoprecipitation: HeLa cell lysate were immunoprecipitated and stained with ARG54113 anti-CDX2 antibody.
客户反馈
Excellent
anti-CDX2 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
anti-CDX2 antibody
Application：WB
Sample：NIH3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Average
anti-CDX2 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
