ARG45179
anti-CLDN16 / Claudin 16 antibody
anti-CLDN16 / Claudin 16 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CLDN16 / Claudin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CLDN16 / Claudin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to C-terminal region of human CLDN16 / Claudin. |
| 偶联标记 | Un-conjugated |
| 別名 | CLDN16; Claudin 16; PCLN1; Paracellin-1; HOMG3; Hypomagnesemia 3, With Hypercalciuria And Nephrocalcinosis; Claudin-16; PCLN-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 34 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLDN16 |
| 全名 | Claudin 16 |
| 背景介绍 | Claudin-16 is a protein that in humans is encoded by the CLDN16 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. |
| 生物功能 | Forms paracellular channels: coassembles with CLDN19 into tight junction strands with cation-selective channels through the strands, conveying epithelial permeability in a process known as paracellular tight junction permeability. [UniProt] |
| 细胞定位 | Cell junction; Cell membrane [UniProt] |
| 预测分子量 | 26 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG45179 anti-CLDN16 / Claudin 16 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45179 anti-CLDN16 / Claudin 16 antibody at 5 ug/ml dilution.
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ARG45179 anti-CLDN16 / Claudin 16 antibody FACS image
Flow Cytometry: PC-3 stained with ARG45179 anti-CLDN16 / Claudin 16 antibody at 1 µg/10^6 cells dilution.
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ARG45179 anti-CLDN16 / Claudin 16 antibody WB image
Western blot: NRK stained with ARG45179 anti-CLDN16 / Claudin 16 antibody at 0.5 μg/ml dilution.
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ARG45179 anti-CLDN16 / Claudin 16 antibody WB image
Western blot: Mouse kidney stained with ARG45179 anti-CLDN16 / Claudin 16 antibody at 0.5 μg/ml dilution.
