ARG23646

anti-CLEC7A antibody [GE2]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
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产品说明书

概述

产品描述	Mouse Monoclonal antibody [GE2] recognizes CLEC7A. This product recognizes human Dectin-1, also known as beta-glucan receptor (beta GR), a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells.Human Dectin-1 has two major (beta GR-A and beta GR-B) and several minor isoforms. beta GR-A and beta GR-B differs by the presence and absence, respectively, of a stalk region. Mouse anti Human Dectin-1 antibody, clone GE2 recognizes both major isoforms. Clone GE2 could be used to inhibit receptor function.
反应物种	Hu
应用	FACS
宿主	Mouse
克隆	Monoclonal
克隆号	GE2
同位型	IgG1
靶点名称	CLEC7A
抗原物种	Human
抗原	NIH3T3 cells expressing full length CLEC7A.
偶联标记	Un-conjugated
別名	C-type lectin superfamily member 12; CD369; Dectin-1; Dendritic cell-associated C-type lectin 1; CLECSF12; CANDF4; BGR; Beta-glucan receptor; C-type lectin domain family 7 member A; DC-associated C-type lectin 1; DECTIN1

应用说明

应用建议

应用	推荐稀释比
FACS	Neat - 1:10

应用说明

FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS and 0.09% Sodium azide.
抗菌剂	0.09% Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 64581 Human CLEC7A Swiss-port # Q9BXN2 Human C-type lectin domain family 7 member A
基因名称	CLEC7A
全名	C-type lectin domain family 7, member A
背景介绍	This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
生物功能	Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). [UniProt]
预测分子量	28 kDa
翻译后修饰	Phosphorylated on tyrosine residues in response to glucan binding. [UniProt]