ARG23644
anti-CLEC7A antibody [GE2] (low endotoxin)
anti-CLEC7A antibody [GE2] (low endotoxin) for Flow cytometry,Functional study and Human
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [GE2] recognizes CLEC7A. This product recognizes human Dectin-1, also known as beta-glucan receptor (beta GR), a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells.Human Dectin-1 has two major (beta GR-A and beta GR-B) and several minor isoforms. beta GR-A and beta GR-B differs by the presence and absence, respectively, of a stalk region. Mouse anti Human Dectin-1 antibody, clone GE2 recognizes both major isoforms. Clone GE2 could be used to inhibit receptor function. |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GE2 |
| 同位型 | IgG1 |
| 靶点名称 | CLEC7A |
| 抗原物种 | Human |
| 抗原 | NIH3T3 cells expressing full length CLEC7A. |
| 偶联标记 | Un-conjugated |
| 別名 | C-type lectin superfamily member 12; CD369; Dectin-1; Dendritic cell-associated C-type lectin 1; CLECSF12; CANDF4; BGR; Beta-glucan receptor; C-type lectin domain family 7 member A; DC-associated C-type lectin 1; DECTIN1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS. |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BXN2 Human C-type lectin domain family 7 member A |
|---|---|
| 基因名称 | CLEC7A |
| 全名 | C-type lectin domain family 7, member A |
| 背景介绍 | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008] |
| 生物功能 | Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues in response to glucan binding. [UniProt] |
