ARG42498
anti-CLTC / Clathrin heavy chain antibody
anti-CLTC / Clathrin heavy chain antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey
概述
| 产品描述 | Goat Polyclonal antibody recognizes CLTC / Clathrin heavy chain |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CLTC / Clathrin heavy chain |
| 抗原物种 | Human |
| 抗原 | Purified recombinant peptide within aa. 50 to the N-terminus of Human CLTC / Clathrin heavy chain. |
| 偶联标记 | Un-conjugated |
| 別名 | CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | AtT-20 | ||||||
| 实际分子量 | ~ 185 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLTC |
| 全名 | clathrin, heavy chain (Hc) |
| 背景介绍 | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008] |
| 生物功能 | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872). [UniProt] |
| 细胞定位 | Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles. [UniProt] |
| 预测分子量 | 192 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42498 anti-CLTC / Clathrin heavy chain antibody ICC/IF image
Immunofluorescence: Hepa1-6 cells were fixed with methanol. Cells were stained with ARG42498 anti-CLTC / Clathrin heavy chain antibody (green) at 1:50 dilution. Nuclear staining (blue).
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ARG42498 anti-CLTC / Clathrin heavy chain antibody WB image
Western blot: 100 µg of AtT-20 cell lysate stained with ARG42498 anti-CLTC / Clathrin heavy chain antibody at 1:500 dilution.
