ARG53919
anti-CLTC / Clathrin heavy chain antibody [BF-06]
anti-CLTC / Clathrin heavy chain antibody [BF-06] for ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Pig
Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [BF-06] recognizes CLTC / Clathrin heavy chain |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Pig |
| 应用 | ELISA, FACS, ICC/IF, IP, WB |
| 特异性 | The clone BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BF-06 |
| 同位型 | IgM |
| 靶点名称 | CLTC / Clathrin heavy chain |
| 偶联标记 | Un-conjugated |
| 別名 | CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | FACS: Human blood leukocytes |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by precipitation methods and ion exchange chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLTC |
| 全名 | clathrin, heavy chain (Hc) |
| 背景介绍 | Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis. |
| 生物功能 | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1) Related products: Clathrin antibodies; Clathrin Duos / Panels; Anti-Mouse IgM secondary antibodies; Related poster download: Organelle Markers & Loading Control |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Endosome Marker antibody |
| 预测分子量 | 192 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: Human primary fibroblasts stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue). -
ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53919 anti-CLTC / Clathrin heavy chain antibody [BF-06] (green).
Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
文献引用
Synaptic endocytosis in adult adipose stromal cell-derived neurons
ICC/IF / Human
