ARG42101
anti-CNTN1 / Contactin 1 antibody
anti-CNTN1 / Contactin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes CNTN1 / Contactin 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CNTN1 / Contactin 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human CNTN1 / Contactin 1. (C-QVTSQEYSARLEN) (NP_001834.2; NP_778203.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Contactin-1; F3; Glycoprotein gp135; GP135; Neural cell surface protein F3; MYPCN |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 140 - 150 kDa (glycosylated) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CNTN1 |
| 全名 | contactin 1 |
| 背景介绍 | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| 生物功能 | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt] |
| 预测分子量 | 113 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG42101 anti-CNTN1 / Contactin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse cerebral cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 5 µg/ml dilution followed by DAB-staining.
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ARG42101 anti-CNTN1 / Contactin 1 antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG42101 anti-CNTN1 / Contactin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42101 anti-CNTN1 / Contactin 1 antibody WB image
Western blot: 35 µg of Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG42101 anti-CNTN1 / Contactin 1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
