ARG41348
anti-COP1 / RFWD2 antibody
anti-COP1 / RFWD2 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes COP1 / RFWD2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | COP1 / RFWD2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 672-701 of Human COP1 / RFWD2. |
| 偶联标记 | Un-conjugated |
| 別名 | RING finger and WD repeat domain protein 2; EC 6.3.2.-; hCOP1; Constitutive photomorphogenesis protein 1 homolog; COP1; E3 ubiquitin-protein ligase RFWD2; RNF200; RING finger protein 200 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | SK-BR-3 | ||||
| 实际分子量 | ~ 90 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RFWD2 |
| 全名 | ring finger and WD repeat domain 2, E3 ubiquitin protein ligase |
| 生物功能 | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. [UniProt] |
| 细胞定位 | Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles. [UniProt] |
| 预测分子量 | 80 kDa |
| 翻译后修饰 | Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41348 anti-COP1 / RFWD2 antibody WB image
Western blot: 35 µg of SK-BR-3 cell lysate stained with ARG41348 anti-COP1 / RFWD2 antibody.
