ARG57153
anti-COX5A antibody [7A6]
anti-COX5A antibody [7A6] for ICC/IF,Western blot and Human
概述
产品描述 | Mouse Monoclonal antibody [7A6] recognizes COX5A |
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反应物种 | Hu |
应用 | ICC/IF, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 7A6 |
同位型 | IgG1, kappa |
靶点名称 | COX5A |
抗原物种 | Human |
抗原 | Recombinant fragment around aa. 42-150 of Human COX5A |
偶联标记 | Un-conjugated |
別名 | Cytochrome c oxidase subunit 5A, mitochondrial; VA; COX-VA; Cytochrome c oxidase polypeptide Va; COX |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purification with Protein A. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 |
Swiss-port # P20674 Human Cytochrome c oxidase subunit 5A, mitochondrial |
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基因名称 | COX5A |
全名 | cytochrome c oxidase subunit Va |
背景介绍 | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008] |
生物功能 | This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
细胞定位 | Mitochondrion inner membrane. [UniProt] |
预测分子量 | 17 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57153 anti-COX5A antibody [7A6] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57153 anti-COX5A antibody [7A6] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57153 anti-COX5A antibody [7A6] WB image
Western blot: 40 µg of 1) A431, and 2) HeLa cell lysates stained with ARG57153 anti-COX5A antibody [7A6] at 1:1000.