ARG42745
anti-CTHRC1 antibody
anti-CTHRC1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CTHRC1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CTHRC1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to S31-K243 of Human CTHRC1. |
| 偶联标记 | Un-conjugated |
| 別名 | Protein NMTC1; Collagen triple helix repeat-containing protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTHRC1 |
| 全名 | collagen triple helix repeat containing 1 |
| 背景介绍 | This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012] |
| 生物功能 | May act as a negative regulator of collagen matrix deposition. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG42745 anti-CTHRC1 antibody ICC/IF image
Immunofluorescence: Hepa 1-6 cells stained with ARG42745 anti-CTHRC1 antibody (red) at 4 μg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42745 anti-CTHRC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42745 anti-CTHRC1 antibody at 1 μg/ml dilution, overnight at 4°C.
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ARG42745 anti-CTHRC1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat lung, Rat thymus, PC-12, Mouse thymus, Raw264.7, SP2/0 and Jurkat whole cell lysates stained with ARG42745 anti-CTHRC1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42745 anti-CTHRC1 antibody FACS image
Flow Cytometry: HepG2 cells were blocked with 10% normal goat serum and then stained with ARG42745 anti-CTHRC1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
