ARG56153
anti-CXCL1 / GRO alpha antibody
anti-CXCL1 / GRO alpha antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Mouse
参考文献2
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXCL1 / GRO alpha |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ELISA, IHC-P, Neut, WB |
| 特异性 | This antibody has been tested by ELISA to have less than 1% cross-reactivity with 50 ng/ml of human CXCL2 (GRO beta) and human CXCL3 (GRO gamma). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXCL1 / GRO alpha |
| 抗原物种 | Human |
| 抗原 | Recombinant Human CXCL1 protein (ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN) |
| 偶联标记 | Un-conjugated |
| 別名 | Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; C-X-C motif chemokine 1; Neutrophil-activating protein 3; GRO1; MGSA; IL-8 homologues (murine) |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Sodium citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCL1 |
| 全名 | chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| 背景介绍 | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014] |
| 生物功能 | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt] |
| 预测分子量 | 11 kDa |
| 翻译后修饰 | N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG56153 anti-CXCL1 / GRO alpha antibody IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon/rectum adenocarcinoma stained with ARG56153 anti-CXCL1 / GRO alpha antibody at 1.5 - 5 µg/ml dilution (overnight, 4°C).
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ARG56153 anti-CXCL1 / GRO alpha antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human GRO protein stained with ARG56153 anti-CXCL1 / GRO alpha antibody. (Reduced condition).
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ARG56153 anti-CXCL1 / GRO alpha antibody ELISA image
ARG56153 anti-CXCL1 / GRO alpha antibody results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG56153 anti-CXCL1 / GRO alpha antibody IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon/rectum adenocarcinoma stained with ARG56153 anti-CXCL1 / GRO alpha antibody at 1.5 - 5 µg/ml µg/ml dilution (overnight, 4°C).
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ARG56153 anti-CXCL1 / GRO alpha antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human GRO protein stained with ARG56153 anti-CXCL1 / GRO alpha antibody. (Unreduced condition).
文献引用
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines.
Neutralize / Mouse
Therapeutic Efficacy of Subcutaneous and Intraperitoneal Injections of a Single Dose of Human Umbilical Mesenchymal Stem Cells in Acute and Chronic Colitis in a Mouse Model.
IHC-P / Mouse
