ARG56693

anti-CXCL3 / GRO gamma antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes CXCL3 / GRO gamma
反应物种	Hu, Ms
应用	ELISA, IHC-P, Neut, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	CXCL3 / GRO gamma
抗原物种	Human
抗原	E.coli derived Recombinant Human GRO gamma. (ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN)
偶联标记	Un-conjugated
別名	Macrophage inflammatory protein 2-beta; MIP2-beta; SCYB3; GRO-gamma; Growth-regulated protein gamma; 5-73; GROg; 1-73; MIP2B; C-X-C motif chemokine 3; CINC-2b; GRO3; MIP-2b

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 0.5 - 2.0 µg/ml with ARG56802 as a detection antibody
IHC-P	0.5 - 0.7 µg/ml
Neut	1.0 - 2.0 µg/ml (To yield [ND50] of the biological activity of Human GRO - γ (100 ng/ml) )
WB	0.1 - 0.2 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2921 Human CXCL3 GeneID: 330122 Mouse CXCL3 Swiss-port # P19876 Human C-X-C motif chemokine 3 Swiss-port # Q6W5C0 Mouse C-X-C motif chemokine 3
基因名称	CXCL3
全名	chemokine (C-X-C motif) ligand 3
背景介绍	This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]
生物功能	Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. [UniProt]
预测分子量	11 kDa
翻译后修饰	N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

检测图片 (5) Click the Picture to Zoom In

ARG56693 anti-CXCL3 / GRO gamma antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma. The recommended ARG56693 anti-CXCL3 / GRO gamma antibody concentration is 0.7 µg/ml - 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56693 anti-CXCL3 / GRO gamma antibody WB image

Western blot: 250 - 0.24 ng of Human GRO gamma stained with ARG56693 anti-CXCL3 / GRO gamma antibody, under reducing conditions.
ARG56693 anti-CXCL3 / GRO gamma antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma. The recommended ARG56693 anti-CXCL3 / GRO gamma antibody concentration is 0.7 µg/ml - 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56693 anti-CXCL3 / GRO gamma antibody WB image

Western blot: 250 - 0.24 ng of Human GRO gamma stained with ARG56693 anti-CXCL3 / GRO gamma antibody, under non-reducing conditions.
ARG56693 anti-CXCL3 / GRO gamma antibody standard curve image

Sandwich ELISA: ARG56693 anti-CXCL3 / GRO gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56802 anti-CXCL3 / GRO gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.