ARG43362
anti-CXCL4 / PF4 antibody
anti-CXCL4 / PF4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXCL4 / PF4 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXCL4 / PF4 |
| 抗原物种 | Rat |
| 抗原 | Recombinant protein corresponding to V30-S105 of Rat CXCL4 / PF4. |
| 偶联标记 | Un-conjugated |
| 別名 | Platelet factor 4; Iroplact; CXCL4; C-X-C motif chemokine 4; Oncostatin-A; SCYB4; PF-4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Rat spleen | ||||||
| 实际分子量 | ~ 11 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PF4 |
| 全名 | platelet factor 4 |
| 背景介绍 | This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014] |
| 生物功能 | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 11 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG43362 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43362 anti-CXCL4 / PF4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43362 anti-CXCL4 / PF4 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat spleen lysate stained with ARG43362 anti-CXCL4 / PF4 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43362 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43362 anti-CXCL4 / PF4 antibody at 1 µg/ml dilutioin, overnight at 4°C.
