ARG66029

anti-CXCL5 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes CXCL5
反应物种	Hu
应用	ELISA, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	CXCL5
抗原物种	Human
抗原	E. coli derived recombinant around aa. 5-78 of Human CXCL5. (AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN)
偶联标记	Un-conjugated
別名	Neutrophil-activating peptide ENA-78; Epithelial-derived neutrophil-activating protein 78; SCYB5; Small-inducible cytokine B5; C-X-C motif chemokine 5; 9-78; 8-78; 1-78; ENA-78

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 0.5 - 2.0 µg/ml with ARG66030 as a detection antibody
IHC-P	1 - 2 µg/ml
WB	0.1 - 0.2 µg/ml

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 6374 Human CXCL5 Swiss-port # P42830 Human C-X-C motif chemokine 5
基因名称	CXCL5
全名	chemokine (C-X-C motif) ligand 5
背景介绍	This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]
生物功能	Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. [UniProt]
预测分子量	12 kDa
翻译后修饰	N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

检测图片 (5) Click the Picture to Zoom In

ARG66029 anti-CXCL5 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon and rectum adenocarcinoma. The recommended ARG66029 anti-CXCL5 antibody concentration is 1.0 µg/ml - 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG66029 anti-CXCL5 antibody WB image

Western blot: 250 - 0.24 ng of Human ENA-78 stained with ARG66029 anti-CXCL5 antibody, under reducing conditions.
ARG66029 anti-CXCL5 antibody standard curve image

Sandwich ELISA: ARG66029 anti-CXCL5 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66030 anti-CXCL5 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
ARG66029 anti-CXCL5 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon and rectum adenocarcinoma. The recommended ARG66029 anti-CXCL5 antibody concentration is 1.0 µg/ml - 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG66029 anti-CXCL5 antibody WB image

Western blot: 250 - 0.24 ng of Human ENA-78 stained with ARG66029 anti-CXCL5 antibody, under non-reducing conditions.