ARG58817
anti-CXCR3 antibody
anti-CXCR3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CXCR3 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CXCR3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to M1-L368 of Human CXCR3. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD183; Interferon-inducible protein 10 receptor; Mig-R; GPR9; MigR; IP10-R; CXCR-3; G protein-coupled receptor 9; CKR-L2; CD183; CD182; CXC-R3; IP-10 receptor; C-X-C chemokine receptor type 3; CMKAR3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CXCR3 |
| 全名 | chemokine (C-X-C motif) receptor 3 |
| 背景介绍 | This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011] |
| 生物功能 | Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Binds to CCL21. Probably promotes cell chemotaxis response. Isoform 2: Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway. Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis. Isoform 3: Mediates the activity of CXCL11. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction. N-glycosylated. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG58817 anti-CXCR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG58817 anti-CXCR3 antibody.
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ARG58817 anti-CXCR3 antibody WB image
Western blot: 40 µg of COLO320 and SGC cell lysates stained with ARG58817 anti-CXCR3 antibody at 0.5 µg/ml dilution.
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ARG58817 anti-CXCR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG58817 anti-CXCR3 antibody.
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ARG58817 anti-CXCR3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG58817 anti-CXCR3 antibody.
