ARG55222
anti-CYC1 antibody
anti-CYC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CYC1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CYC1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 142-176 (Center) of Human CYC1. |
| 偶联标记 | Un-conjugated |
| 別名 | Cytochrome c1, heme protein, mitochondrial; UQCR4; Cytochrome b-c1 complex subunit 4; Complex III subunit 4; Complex III subunit IV; Cytochrome c-1; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; MC3DN6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08574 Human Cytochrome c1, heme protein, mitochondrial Swiss-port # Q9D0M3 Mouse Cytochrome c1, heme protein, mitochondrial |
|---|---|
| 基因名称 | CYC1 |
| 全名 | cytochrome c-1 |
| 背景介绍 | This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013] |
| 生物功能 | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. [UniProt] |
| 细胞定位 | Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side |
| 研究领域 | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Binds 1 heme group per subunit. |
检测图片 (3) Click the Picture to Zoom In
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ARG55222 anti-CYC1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55222 anti-CYC1 antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG55222 anti-CYC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG55222 anti-CYC1 antibody at 1:25 dilution.
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ARG55222 anti-CYC1 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55222 anti-CYC1 antibody at 1:1000 dilution.
客户反馈
Excellent
anti-CYC1 antibody
Application：WB
Sample：HeLa and MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
