ARG22260
anti-CaMKII alpha antibody [6G9]
anti-CaMKII alpha antibody [6G9] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Mouse,Rat,Bovine
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [6G9] recognizes CaMKII alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 应用 | ELISA, ICC/IF, IHC-P, IP, RIA, WB |
| 特异性 | Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6G9 |
| 同位型 | IgG1 |
| 靶点名称 | CaMKII alpha |
| 抗原物种 | Rat |
| 抗原 | Partially purified Rat CaMKII |
| 偶联标记 | Un-conjugated |
| 別名 | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Camk2a |
| 全名 | calcium/calmodulin-dependent protein kinase II alpha |
| 背景介绍 | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| 生物功能 | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, Cell Junction, Mitochondrion, Nucleus, Presynaptic Cell Membrane, Synapse |
| 预测分子量 | 54 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG22260 anti-CaMKII alpha antibody [6G9] ICC/IF image
Immunocytochemistry: Mouse dissociated hippocampal neurons stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.
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ARG22260 anti-CaMKII alpha antibody [6G9] WB image
Western blot: Rat brain lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9].
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ARG22260 anti-CaMKII alpha antibody [6G9] IHC image
Immunohistochemistry: Formalin fixed colon carcinoma stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:10000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.
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ARG22260 anti-CaMKII alpha antibody [6G9] WB image
Western blot: Rat brain membrane lysate stained with ARG22260 anti-CaMKII alpha antibody [6G9] at 1:1000 dilution.
文献引用
Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats
WB / Rat
