ARG22220
anti-Calreticulin antibody
anti-Calreticulin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Guinea pig,Hamster,Monkey,Pig,Rabbit,Sheep
Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Calreticulin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Mk, Pig, Rb, Sheep |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | Detects ~63kDa. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 靶点名称 | Calreticulin |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide from Human calreticulin (with a cysteine residue added) |
| 偶联标记 | Un-conjugated |
| 別名 | CALR; Calreticulin; Calregulin; CC1qR; SSA; CRT; RO; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; FLJ26680; CALR1; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n; Autoantigen Ro; HEL-S-99n; CRTC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CALR |
| 全名 | calreticulin |
| 背景介绍 | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type. |
| 生物功能 | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. |
| 细胞定位 | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Lysosome, Sarcoplasmic reticulum, Secreted |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Acetylation, Disulfide bond, Glycoprotein, Hydroxylation |
检测图片 (4) Click the Picture to Zoom In
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ARG22220 anti-Calreticulin antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22220 anti-Calreticulin antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22220 anti-Calreticulin antibody IHC image
Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22220 anti-Calreticulin antibody (green) at 1:100 dilution (1 hour).
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ARG22220 anti-Calreticulin antibody WB image
Western blot: 15 µg of A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3, T98G, and Rat brain lysates stained with ARG22220 anti-Calreticulin antibody at 1:1000 dilution.
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ARG22220 anti-Calreticulin antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22220 anti-Calreticulin antibody (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
