ARG10147
anti-Cardiac Troponin T antibody [1C11]
anti-Cardiac Troponin T antibody [1C11] for ELISA,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Mouse,Rat,Dog
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [1C11] recognizes Cardiac Troponin T |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog |
| 应用 | ELISA, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, IP |
| 特异性 | No cross-reaction with skeletal TnT. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1C11 |
| 同位型 | IgG1 |
| 靶点名称 | Cardiac Troponin T |
| 抗原物种 | Human |
| 抗原 | free human cTnT |
| 偶联标记 | Un-conjugated |
| 別名 | RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) for 5 - 10 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A affinity purified. |
| 缓冲液 | PBS (pH 7.4) and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 浓度 | 1.0-2.0 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TNNT2 |
| 全名 | troponin T type 2 (cardiac) |
| 背景介绍 | The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG10147 anti-Cardiac Troponin T antibody [1C11] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse embryo (E9.75) section stained with ARG10147 anti-Cardiac Troponin T antibody [1C11] at 1:150 dilution. Antigen Retrieval: Citrate buffer (pH 6.0) for 5 - 10 min.
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ARG10147 anti-Cardiac Troponin T antibody [1C11] IHC-Fr image
Immunohistochemistry: Frozen section of Human myocardial tissue stained with ARG10147 anti-Cardiac Troponin T antibody [1C11] at 1:300 dilution.
克隆号文献
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
IHC-Fr / Mouse
Superparamagnetic iron oxide nanoparticles function as a long-term, multi-modal imaging label for non-invasive tracking of implanted progenitor cells.
IHC-P / Rat
RhoA/ROCK signaling and pleiotropic α1A-adrenergic receptor regulation of cardiac contractility.
WB / Mouse
Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation.
IHC-P / Mouse
Fetal reprogramming and senescence in hypoplastic left heart syndrome and in human pluripotent stem cells during cardiac differentiation.
IHC, FACS /
Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
ICC/IF /
