ARG10705
anti-Cas9 (S. aureus) antibody [6F7]
anti-Cas9 (S. aureus) antibody [6F7] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
概述
产品描述 | Mouse Monoclonal antibody [6F7] recognizes Cas9 (S. aureus) |
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反应物种 | Bacteria |
应用 | ICC/IF, IHC-Fr, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 6F7 |
同位型 | IgG1 |
靶点名称 | Cas9 (S. aureus) |
抗原物种 | Bacteria |
抗原 | C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli. |
偶联标记 | Un-conjugated |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Affinity purification. |
缓冲液 | PBS and 50% Glycerol. |
稳定剂 | 50% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
产品亮点 | Related products: Cas9 antibodies; Anti-Mouse IgG secondary antibodies; Related news: How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer |
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检测图片 (2) Click the Picture to Zoom In
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ARG10705 anti-Cas9 (S. aureus) antibody [6F7] ICC/IF image
Immunocytochemistry: Transfected HEK293 cells with GFP-Cas9 (SA) fusion protein were stained with ARG10705 anti-Cas9 (S. aureus) antibody [6F7] (red) at 1:1000 dilution. GFP is expressed in the transfected cells (green). Only transfected cells are reactive with ARG10705 anti-Cas9 (S. aureus) antibody [6F7] antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
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ARG10705 anti-Cas9 (S. aureus) antibody [6F7] WB image
Western blot: 1) HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10705 anti-Cas9 (S. aureus) antibody [6F7] at 1:1000 dilution.