ARG10681
anti-Cas9 (S. aureus) antibody
anti-Cas9 (S. aureus) antibody for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
概述
| 产品描述 | Chicken Polyclonal antibody recognizes Cas9 (S. aureus) |
|---|---|
| 反应物种 | Bacteria |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | Cas9 (S. aureus) |
| 抗原物种 | Bacteria |
| 抗原 | C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 产品亮点 | Related products: Cas9 antibodies; Anti-Chicken IgY secondary antibodies; Related news: How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer |
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检测图片 (2) Click the Picture to Zoom In
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ARG10681 anti-Cas9 (S. aureus) antibody ICC/IF image
Immunocytochemistry: Transfected HEK293 cells which overexpressing GFP-Cas9 (SA) fusion protein were stained with ARG10681 anti-Cas9 (S. aureus) antibody. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with ARG10681 anti-Cas9 (S. aureus) antibody is shown in red in middle panel. In merged image (right panel), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
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ARG10681 anti-Cas9 (S. aureus) antibody WB image
Western blot: 1) HEK293 cells overexpressing a fusion protein containing GFP and the C-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10681 anti-Cas9 (S. aureus) antibody at 1:1000 dilution.
