ARG10706
anti-Cas9 (S. pyogenes) antibody [3F9]
anti-Cas9 (S. pyogenes) antibody [3F9] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
概述
| 产品描述 | Mouse Monoclonal antibody [3F9] recognizes Cas9 (S. pyogenes) |
|---|---|
| 反应物种 | Bacteria |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3F9 |
| 同位型 | IgG |
| 靶点名称 | Cas9 (S. pyogenes) |
| 抗原物种 | Bacteria |
| 抗原 | N-terminal region, aa. 1-608 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 产品亮点 | Related products: Cas9 antibodies; Anti-Mouse IgG secondary antibodies; Related news: How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer |
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检测图片 (2) Click the Picture to Zoom In
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ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] ICC/IF image
Immunocytochemistry: Transfected HEK293 cells overexpressing the N-terminal aa. 1-608 of S. pyogenes Cas9. The cells were stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] (red), and these cells also co-stained with ARG10734 anti-Cas9 antibody to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most HEK293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.
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ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] WB image
Western blot: 1) MW marker, 2) crude protein extract from Hek293 culture transfected with the immunogen, the N-terminal 1-608 amino acids, 3) Non-transfected control Hek293 cell extract, and 4) 40 ng of Streptococcus pyogenes Cas9 (full length) was stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] at 1:1000 dilution.
