ARG23707
anti-Caspase 1 antibody
anti-Caspase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Caspase 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | The antibody might react full length and p10 cleaved from of caspase 1 protein based on immunogen sequence design. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Caspase 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 348-361 within the p10 subunit of Human Caspase-1. (VFIGRLIEHMQEYA) |
| 偶联标记 | Un-conjugated |
| 別名 | Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP1 |
| 全名 | caspase 1, apoptosis-related cysteine peptidase |
| 背景介绍 | Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| 生物功能 | Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt] |
| 产品亮点 | Related products: Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH |
| 研究领域 | Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG23707 anti-Caspase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG23707 anti-Caspase 1 antibody.
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ARG23707 anti-Caspase 1 antibody WB image
Western blot: 1) MCF-7, 2) HeLa and 3) SW620 cell lysate stained with ARG23707 anti-Caspase 1 antibody at 0.5 µg/ml dilution.
