ARG66328
anti-Caspase 3 antibody [SQab1876]
anti-Caspase 3 antibody [SQab1876] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1876] recognizes Caspase 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1876 |
| 同位型 | IgG |
| 靶点名称 | Caspase 3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 1-100 of Human Caspase 3. |
| 偶联标记 | Un-conjugated |
| 別名 | CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP3 |
| 全名 | caspase 3, apoptosis-related cysteine peptidase |
| 背景介绍 | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| 生物功能 | Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| 预测分子量 | 32 kDa, 17 kDa (cleaved) |
| 翻译后修饰 | Acetylation, Phosphoprotein, S-nitrosylation, Zymogen. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66328 anti-Caspase 3 antibody [SQab1876] ICC/IF image
Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:800 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66328 anti-Caspase 3 antibody [SQab1876] FACS image
Flow Cytometry: Ramos cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66328 anti-Caspase 3 antibody [SQab1876] WB image
Western blot: 20 µg of Jurkat treated with 1 µM staurosporine for 4h (+) or untreated (-). The blots were stained with ARG66328 anti-Caspase 3 antibody [SQab1876] at 1:2000 dilution.
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ARG66328 anti-Caspase 3 antibody [SQab1876] IP image
Immunoprecipitation: 0.4 mg of Jurkat whole cell lysate immunoprecipitated (1:50) and stained with ARG66328 anti-Caspase 3 antibody [SQab1876]. 1) ARG66328 IP in Jurkat whole cell lysate, 2) PBS instead of ARG66328 in Jurkat whole cell lysate, and 3) Jurkat whole cell lysate, 10 µg (input).
