ARG42743
anti-Catalase antibody
anti-Catalase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Catalase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Catalase |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to E344-L527 of Human Catalase. |
| 偶联标记 | Un-conjugated |
| 別名 | Catalase; EC 1.11.1.6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | ~ 57 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CAT |
| 全名 | catalase |
| 背景介绍 | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009] |
| 生物功能 | Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. [UniProt] |
| 细胞定位 | Peroxisome. [UniProt] |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | The N-terminus is blocked. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG42743 anti-Catalase antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG42743 anti-Catalase antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
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ARG42743 anti-Catalase antibody WB image
Western blot: Rat skeletal muscle, Mouse skeletal muscle and A549 whole cell lysates stained with ARG42743 anti-Catalase antibody at 0.5 µg/ml dilution.
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ARG42743 anti-Catalase antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG42743 anti-Catalase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
