ARG64743
anti-Clathrin light chain antibody
anti-Clathrin light chain antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Clathrin light chain |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all reported isoforms of light chains a and b. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Clathrin light chain |
| 抗原物种 | Human |
| 抗原 | CDFNPKSSKQAKD |
| 偶联标记 | Un-conjugated |
| 別名 | Clathrin light chain A; Lca; LCA |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLTA |
| 全名 | clathrin, light chain A |
| 背景介绍 | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010] |
| 生物功能 | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. [UniProt] |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 27 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64743 anti-Clathrin light chain antibody WB image
Western blot: 35 µg of Human Frontal Cortex lysate stained with ARG64743 anti-Clathrin light chain antibody at 0.1 µg/ml dilution.
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ARG64743 anti-Clathrin light chain antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64743 anti-Clathrin light chain antibody at 2.5 µg/ml dilution followed by AP-staining.
