ARG66489
anti-Collagen III antibody
anti-Collagen III antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Collagen III |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Collagen III |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human Collagen III. |
| 偶联标记 | Un-conjugated |
| 別名 | EDS4A; Collagen alpha-1(III) chain |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citric acid buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COL3A1 |
| 全名 | collagen, type III, alpha 1 |
| 背景介绍 | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| 生物功能 | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 139 kDa |
| 翻译后修饰 | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66489 anti-Collagen III antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skin stained with ARG66489 anti-Collagen III antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
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ARG66489 anti-Collagen III antibody WB image
Western blot: 30 µg of HeLa whole cell lysate stained with ARG66489 anti-Collagen III antibody at 1:1000 dilution.
