ARG20786
anti-Collagen III antibody, pre-adsorbed
anti-Collagen III antibody, pre-adsorbed for Electron microscopy,ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Chicken,Dog,Hamster,Human,Mouse,Pig,Rabbit,Rat
参考文献10
概述
| 产品描述 | Goat Polyclonal antibody recognizes Collagen III |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Chk, Dog, Hm, Pig, Rb |
| 应用 | ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | The antibody reacts with conformational determinants on type III collagen. The antibody is pre-adsorbed with Collagen types I, II, IV, V and VI, so the antibody may not react with Collagen types I, II, IV, V and VI. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Collagen III |
| 抗原物种 | Human |
| 抗原 | Human Type III collagen |
| 偶联标记 | Un-conjugated |
| 別名 | EDS4A; Collagen alpha-1(III) chain |
应用说明
| 预吸附 | Collagen types I, II, IV, V and VI. | ||||||||||||||||
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| 应用建议 |
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| 应用说明 | WB: The antibody reacts with native and denature form of Collagen III protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | BBS (pH 8.2) |
| 浓度 | 0.4 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COL3A1 |
| 全名 | collagen, type III, alpha 1 |
| 背景介绍 | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| 生物功能 | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt] |
| 预测分子量 | 139 kDa |
| 翻译后修饰 | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. |
检测图片 (4) Click the Picture to Zoom In
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ARG20786 anti-Collagen III antibody, pre-adsorbed ICC/IF image
Immunofluorescence: Rabbit bone marrow stromal cells stained with ARG20786 anti-Collagen III antibody, pre-adsorbed.
From Cheng-Chang Lu et al. Bone Joint Res. (2023), doi: 10.1302/2046-3758.121.BJR-2021-0434.R2, Fig. 6.
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ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.
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ARG20786 anti-Collagen III antibody, pre-adsorbed WB image
Western blot: Embryonic chick cardiac tissue samples separated by SDS-PAGE and stained with ARG20786 anti-Collagen III antibody (pre-adsorbed) under reducing conditions.
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ARG20786 anti-Collagen III antibody (pre-adsorbed) IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.
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