ARG42686
anti-Collagen IV alpha 2 antibody
anti-Collagen IV alpha 2 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Collagen IV alpha 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Hm |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Collagen IV alpha 2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 1697-1712 of Human Collagen IV alpha 2. (LIRTHISRCQVCMKNL) |
| 偶联标记 | Un-conjugated |
| 別名 | ICH; POREN2; Collagen alpha-2(IV) chain |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COL4A2 |
| 全名 | collagen, type IV, alpha 2 |
| 背景介绍 | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008] |
| 生物功能 | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix, basement membrane. [UniProt] |
| 预测分子量 | 168 kDa |
| 翻译后修饰 | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. Proteolytic processing produces the C-terminal NC1 peptide, canstatin. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG42686 anti-Collagen IV alpha 2 antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG42686 anti-Collagen IV alpha 2 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42686 anti-Collagen IV alpha 2 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat kidney tissue stained with ARG42686 anti-Collagen IV alpha 2 antibody.
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ARG42686 anti-Collagen IV alpha 2 antibody WB image
Western blot: Rat kidney and Rat lung lysates stained with ARG42686 anti-Collagen IV alpha 2 antibody.
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ARG42686 anti-Collagen IV alpha 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG42686 anti-Collagen IV alpha 2 antibody.
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ARG42686 anti-Collagen IV alpha 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42686 anti-Collagen IV alpha 2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42686 anti-Collagen IV alpha 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42686 anti-Collagen IV alpha 2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42686 anti-Collagen IV alpha 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42686 anti-Collagen IV alpha 2 antibody at 1 µg/ml dilution, overnight at 4°C.
