ARG21972

anti-Collagen IV antibody [2F11]

anti-Collagen IV antibody [2F11] for ELISA,FLISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human

Angiogenesis Study antibody; Basement Membrane Marker antibody

概述

产品描述 Mouse Monoclonal antibody [2F11] recognizes Collagen IV
反应物种 Hu
应用 ELISA, FLISA, IHC-Fr, IHC-P
特异性 Human type IV collagen
宿主 Mouse
克隆 Monoclonal
克隆号 2F11
同位型 IgG1, kappa
靶点名称 Collagen IV
抗原物种 Human
抗原 Native Human type IV collagen
偶联标记 Un-conjugated
別名 BSVD; RATOR; Collagen alpha-1(IV) chain

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
FLISAAssay-dependent
IHC-Fr< 2 µg/ml
IHC-PAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 BBS (pH 8.2)
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1282 Human COL4A1

Swiss-port # P02462 Human Collagen alpha-1(IV) chain

基因名称 COL4A1
全名 collagen, type IV, alpha 1
背景介绍 Collagen IV proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
生物功能 Collagen IV is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. [UniProt]
产品亮点 Related products:
Collagen IV antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Vascular development is regulated by FGF-dependent metabolic control
研究领域 Angiogenesis Study antibody; Basement Membrane Marker antibody
预测分子量 161 kDa
翻译后修饰 Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten.

检测图片 (2) Click the Picture to Zoom In

  • ARG21972 anti-Collagen IV antibody [2F11] IHC-Fr image

    Immunohistochemistry: Frozen section of Human skin tissue stained with ARG21972 anti-Collagen IV antibody [2F11] followed by Goat anti-Mouse IgG1 antibody (Alexa Fluor® 488) and DAPI.

  • ARG21972 anti-Collagen IV antibody [2F11] IHC-Fr image

    Immunohistochemistry: Frozen section of Human skin tissue stained with anti-Type I Collagen antibody and ARG21972 anti-Collagen IV antibody [2F11] followed by ARG21895 Goat anti-Mouse IgG2b antibody (Biotin) (pre-adsorbed), Goat anti-Mouse IgG1 antibody (Alexa Fluor® 488), Streptavidin (Cy3) and DAPI.