ARG52923
anti-Cyclin D1 antibody [SP4]
anti-Cyclin D1 antibody [SP4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
参考文献4
概述
| 产品描述 | Rabbit Monoclonal antibody [SP4] recognizes Cyclin D1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SP4 |
| 同位型 | IgG |
| 靶点名称 | Cyclin D1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from C-terminus of human cyclin D1. |
| 偶联标记 | Un-conjugated |
| 別名 | B-cell lymphoma 1 protein; PRAD1; U21B31; D11S287E; BCL-1; G1/S-specific cyclin-D1; BCL-1 oncogene; BCL1; PRAD1 oncogene |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Mantle Cell Lymphoma, Tonsil, MCF-7 |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). |
| 细胞定位 | Nucleus |
| 产品亮点 | Related products: Cyclin D1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization. |
检测图片 (13) Click the Picture to Zoom In
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ARG52923 anti-Cyclin D1 antibody [SP4] WB image
Western blot: 30 µg of HeLa, SW480, 3T3, and Mouse kidney lysates stained with ARG52923 anti-Cyclin D1 antibody [SP4] at 1:500 dilution.
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ARG52923 anti-Cyclin D1 antibody [SP4] ICC/IF image
Immunofluorescence: H1793 cells were fixed with cold methanol:acetone (1:1) for 5 minutes at -20°C, permeabilized with 0.3% Triton X-100 for 30 min at RT then blocked with 1% goat serum for 30 min at RT. Cells were stained with ARG52923 anti-Cyclin D1 antibody [SP4] (red) at 1:100 dilution for 1 hour at RT and DAPI (blue) was used as the nuclear counter stain.
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human B Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Endometrial Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human HK Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Lung Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Ovarian Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Prostate Adenocarcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Renal Cell Carcinoma stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Tonsil stained with Cyclin D1 antibody [SP4] (ARG52923)
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ARG52923 anti-Cyclin D1 antibody [SP4] IHC-P image
Immunohistochemistry: Human Mantle Cell Lymphoma stained with Cyclin D1 antibody [SP4] (ARG52923)
客户反馈
Excellent
anti-Cyclin D1 antibody [SP4]
Application：WB
Sample：Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
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