ARG64171
anti-Cyld antibody
anti-Cyld antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Cyld |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cyld |
| 抗原物种 | Mouse |
| 抗原 | C-DSQQSKSKNPWYIDE |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin carboxyl-terminal hydrolase CYLD; SBS; Ubiquitin thioesterase CYLD; CYLD1; TEM; USPL2; EAC; Ubiquitin-specific-processing protease CYLD; CDMT; MFT1; MFT; BRSS; CYLDI; Deubiquitinating enzyme CYLD; EC 3.4.19.12 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q66H62 Rat Ubiquitin carboxyl-terminal hydrolase CYLD Swiss-port # Q80TQ2 Mouse Ubiquitin carboxyl-terminal hydrolase CYLD |
|---|---|
| 基因名称 | Cyld |
| 全名 | cylindromatosis (turban tumor syndrome) |
| 背景介绍 | This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B (By similarity). Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation (PubMed:25134987). [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 107 kDa |
| 翻译后修饰 | Phosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B. |
检测图片 (2) Click the Picture to Zoom In
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ARG64171 anti-Cyld antibody WB image
Western Blot: rat brain lysate (35 µg protein in RIPA buffer) stained with ARG64171 anti-Cyld antibody at 0.5 µg/ml dilution.
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ARG64171 anti-Cyld antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64171 anti-Cyld antibody at 5 µg/ml dilution followed by AP-staining.
