ARG62461
anti-Cytochrome C antibody [6H2.B4]
anti-Cytochrome C antibody [6H2.B4] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
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概述
| 产品描述 | Mouse Monoclonal antibody [6H2.B4] recognizes Cytochrome C |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6H2.B4 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Cytochrome C |
| 抗原物种 | Rat |
| 抗原 | Rat cyt c-OVA |
| 偶联标记 | Un-conjugated |
| 別名 | CYC; HCS; Cytochrome c; THC4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | affinity chromatography |
| 缓冲液 | PBS (pH 7.2) and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Cycs |
| 全名 | cytochrome c, somatic |
| 背景介绍 | This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010] |
| 生物功能 | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity). [UniProt] |
| 细胞定位 | Mitochondrial intermembrane space |
| 产品亮点 | Related Antibody Duos and Panels: ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60) ARG30276 Cytochrome-C fractionation Antibody Panel (Cytochrome-C, COX IV, beta Actin) Related products: Cytochrome C antibodies; Cytochrome C Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Understanding Your Cells: Choose the right markers |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Binds 1 heme group per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration. |
检测图片 (1) Click the Picture to Zoom In
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ARG62461 anti-Cytochrome C antibody [6H2.B4] WB image
Western blot: 20 µg of HeLa and Mouse testis lysates stained with ARG62461 anti-Cytochrome C antibody [6H2.B4] at 1:500 dilution.
客户反馈
Excellent
anti-Cytochrome C antibody [6H2.B4]
Application：WB
Sample：Mouse kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-Cytochrome C antibody [6H2.B4]
Application：WB
Sample：Rat kidney lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
