ARG56070
anti-Cytochrome C antibody [7H8.2C12]
anti-Cytochrome C antibody [7H8.2C12] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [7H8.2C12] recognizes Cytochrome C |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 7H8.2C12 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Cytochrome C |
| 抗原 | Synthetic peptides around aa. 1-80, 81-104 and 66-104 of Pigeon Cytochrome C. |
| 偶联标记 | Un-conjugated |
| 別名 | CYC; HCS; Cytochrome c; THC4 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CYCS |
| 全名 | cytochrome c, somatic |
| 背景介绍 | This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010] |
| 生物功能 | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Binds 1 heme group per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration. |
检测图片 (3) Click the Picture to Zoom In
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ARG56070 anti-Cytochrome C antibody [7H8.2C12] WB image
Western blot: 30 µg of Mouse stomach lysate stained with ARG56070 anti-Cytochrome C antibody [7H8.2C12] at 1:200 dilution.
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ARG56070 anti-Cytochrome C antibody [7H8.2C12] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human salivary tumor stained with ARG56070 anti-Cytochrome C antibody [7H8.2C12].
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ARG56070 anti-Cytochrome C antibody [7H8.2C12] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human pancreas stained with ARG56070 anti-Cytochrome C antibody [7H8.2C12].
客户反馈
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anti-Cytochrome C antibody [7H8.2C12]
Application：WB
Sample：Mouse stomach
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
