ARG66336
anti-Cytokeratin 10 antibody [SQab1884]
anti-Cytokeratin 10 antibody [SQab1884] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1884] recognizes Cytokeratin 10 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1884 |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 10 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human Cytokeratin 10. |
| 偶联标记 | Un-conjugated |
| 別名 | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT10 |
| 全名 | keratin 10, type I |
| 背景介绍 | Cytokeratin 10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt] |
| 产品亮点 | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 59 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66336 anti-Cytokeratin 10 antibody [SQab1884] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde for 10 mins. The cells were stained with ARG66336 anti-Cytokeratin 10 antibody [SQab1884] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66336 anti-Cytokeratin 10 antibody [SQab1884] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cervix tissue stained with ARG66336 anti-Cytokeratin 10 antibody [SQab1884] at 1:12,800 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
