ARG62970
anti-Cytokeratin 10 antibody [VIK-10]
anti-Cytokeratin 10 antibody [VIK-10] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [VIK-10] recognizes Cytokeratin 10 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, IP |
| 特异性 | The clone VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | VIK-10 |
| 同位型 | IgG1 |
| 靶点名称 | Cytokeratin 10 |
| 抗原物种 | Human |
| 抗原 | Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction. |
| 偶联标记 | Un-conjugated |
| 別名 | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Pretreatment: Trypsin, at 37°C. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| 阳性对照 | IHC-P: Skin |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT10 |
| 全名 | keratin 10, type I |
| 背景介绍 | Cytokeratin 10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 59 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG62970 anti-Cytokeratin 10 antibody [VIK-10] IHC-P image
Immunohistochemistry: Skin basaliom (paraffin-embedded sections) stained with ARG62970 anti-Cytokeratin 10 antibody [VIK-10].
